To Prime Hook from Norfolk, VA and Southern Delmarva

Take US 13 north from Chesapeake Bay Bridge Tunnel. Continue north into Delaware on US 13 or 113 (Pokomoke, MD) to Delaware Route 16. Take Delaware Route 16 east to Delaware Route 1 east of Milton. Stay on Route 16 going east toward Broadkill Beach. At 1.1 miles east of Delaware Route 1 turn left onto Turkle Pond Road (refuge sign posted). Follow Turkle Pond Road, 1.6 mi to office/visitor center.

To Prime Hook from Washington/Baltimore, MD

From BWI - take I 97 South to US 50. Take US 50 east the to Bay Bridge. After crossing the Bay Bridge continue east on Route 50 to MD 404 (Delaware Beaches/Rehoboth Beach). Take 404 east to State Route 16 (Maryland Rt. 16) east of Denton, MD. Follow Route 16 east to Delaware then Delaware Route 16 east to State Route 1 east of Milton, DE (crossing USA 13 & 113) to Delaware Route 1. Continue on Route 16 east toward Broadkill Beach 1.1 mile east of Route1(see above) turn left onto Turkle Pond Road. (Refuge sign posted). Follow Turkle Pond Road, 1.6 mi to office/visitor center.

To Prime Hook from New Jersey, Philadelphia Area

Follow I-95 south to Delaware 1 (new roadway). Follow Delaware 1 south - the road will be partially limited access, partially old US 13/Delaware 1. Follow south to Dover Air Force Base. At end of expressway follow US 113 south to Milford (+ 15 mi). At Milford Take Route 1 south (Rehoboth Beach). Approximately 15 mi south of Milford the first traffic light is the intersection of Route 1 and Route 16 (refuge sign posted). Turn left on State Route 16. Follow Route 16 east to toward Broadkill Beach. At 1.1 miles east turn left onto Turkle Pond Road. Follow Turkle Pond Road, 1.6 mi to office/visitor center.